GMO Jab warnings from 30 years ago revisited

Endotoxin and Plasmid DNA contamination were top of mind for Klaus Cichutek when he wrote a key review in 1994. He directs the Paul Ehrlich Institute and effectively controls EU GMO Jab development.

I checked Substack and found my friend Dr Sabine Stebel had already written an excellent review of a 1994 paper by Dr Klaus Cichutek.

If you use a browser like Brave, you might like to read her article automatically translated.¹

I won’t duplicate much of Dr Bine’s analysis but thought with the current focus on Monkeypox Jabs, we should remind ourselves that Klaus Cichutek thinks it is a great idea to genetically modify Monkey viruses and use them to alter the Human Genetic code, as he outlined in 1994.²

Note the “Limited Replication by Integrase-negative Mutants of Simian Immunodeficiency Virus” designed to attack, enter and multiply inside Human cells.

Cichutek showed a Human Somatic cell “which may or may not integrate proviral DNA”.

He continued playing with mutating viruses while at the same time steering regulation of GMO.³⁴⁵

Cichutek fascination with Retrotranspons

I might write more about Cichutek’s research into ApolipoProtein B mRNA Editing Catalytic polypeptide-like (APOBEC) proteins, but I know a number of my readers are better qualified on the subject, so I’ll add a few of his papers as references in the meantime.⁶⁷⁸⁹¹⁰¹¹¹²¹³¹⁴¹⁵

“Dysregulation of their functions can cause undesired genomic mutations and RNA modification, leading to various associated diseases, such as hyper-IgM syndrome and cancer.”¹⁶

Endotoxin research under Cichutek

In 1994 Cichutek stated, looking to the future of GMO Humans:

After gene transfer, the gene should not produce pathological effects as such. Adventitious agents and NAs possibly resulting from production should not be present.

Specifically, adventitious NAs and sequences that allow recombination with oncogenes or tumour-suppressor genes should be avoided.

Sequences needed for amplification, e.g. in bacterial cells, should not be expressed in cells.

Impurities in the final product may depend on the manufacturing and purification procedures chosen.

For example, endotoxins, as typical by-products of manufacturing processes using bacterial cells, should be removed. Variability in the cultures during production may lead to variability in the consistency of the final product, and thus procedures ensuring product consistency are imperative.

Scale-up of the production or purification schemes may have significant consequences for the product and thus have to be thoroughly controlled or their effects on the final product.

Endotoxin research funded by German taxpayers has been extensive at PEI, as mentioned earlier.¹⁷¹⁸¹⁹

Here is an example from the 2002 PEI report, where we see PEI was working on Endotoxin removal from patient blood with an external absorber.

New subscribers might like other articles related to Paul Ehrlich Institute in the Klaus Cichutek decades.²⁰²¹²²²³

1. Wie man sich aus seinen eigenen Worten einen Strick dreht weiß der Klausi

2. Cichutek K. Nucleic acid immunization: a prophylactic gene therapy? 1994. Vaccine. 12(16):1520-5. https://www.sciencedirect.com/science/article/abs/pii/0264410X94900760

3. Engelstädter, M. ; Buchholz, C.J. ; Bobkova, M. ; Steidl, S. ; Merget-Millitzer, H. ; Willemsen, R.A. ; Stitz, J. ; Cichutek, K. 2001. Targeted gene transfer to lymphocytes using murine leukaemia virus vectors pseudotyped with spleen necrosis virus envelope proteins. In: Gene Therapy 8(15):1202-1206

4. Schweizer, M. ; Flory, E. ; Buchholz, C.J. ; Cichutek, K.: Retroviral vectors. 2001. In: Bundesgesundheitsblatt. Gesundheitsforschung. Gesundheitsschutz 44(11):1038-1046

5. Stitz, J. ; Mühlebach, M. ; Blömer, U. ; Scherr, M. ; Selbert, M. ; Wehner, P. ; Steidl, S. ; Schmitt, I. ; König, R. ; Schweizer, M. ; Cichutek, K. 2001. A novel lentivirus vector derived from apathogenic simian immunodeficiency virus. In: Virology 291:191-197

6. Cichutek, K. Somatische Gentherapie : Stand der klinischen Forschung und Regularien in Deutschland. In: Eberbach ; Lange ; Ronellenfitsch: Recht der Gentechnik und Biomedizin : GenTR/BioMedR. . Heidelberg : Verl. C.F. Müller, 2001

7. Muckenfuss H, Kaiser JK, Krebil E, Battenberg M, Schwer C, Cichutek K, Münk C, Flory E. 2007. Sp1 and Sp3 regulate basal transcription of the human APOBEC3G gene. Nucleic Acids Res 35: 3784-3796

8. Münk C, Zielonka J, Constabel H, Kloke BP, Rengstl B, Battenberg M, Bonci F, Pistello M, Löchelt M, Cichutek K. 2007. Multiple Restrictions of Human Immunodeficiency Virus Type 1 in Feline Cells. J Virol 81: 7048-7060

9. Wolfrum N, Mühlebach MD, Schüle S, Kaiser JK, Kloke BP, Cichutek K, Schweizer M. 2007. Impact of viral accessory proteins of SIVsmmPBj on early steps of infection of quiescent cells. Virology 364: 330-341

10. Funke S, Maisner A, Mühlebach MD, Koehl U, Grez M, Cattaneo R, Cichutek K, Buchholz CJ 2008. Targeted cell entry of lentiviral vectors. Mol Ther 16: 1427-1436

11. Münk C, Beck T, Zielonka J, Hotz-Wagenblatt A, Chareza S, Battenberg M, Thielebein J, Cichutek K, Bravo IG, O‘Brien SJ, Löchelt M, Yuhki N. 2008. Functions, structure, and read-through alternative splicing of feline APOBEC3 genes. Genome Biol 9: R48

12. Urban, J. H., Schneider, R. M., Compte, M., Finger, C., Cichutek, K., Alvarez-Vallina, L., and Buchholz, C. J. 2005. Selection of functional human antibodies from retroviral display libraries. Nucleic Acids Res 33, e35.

13. Stauch, B.; Hofmann, H.; Perkovic, M.; Weisel, M.; Kopietz, F.; Cichutek, K.; Münk, C.; Schneider, G. Model structure of APOBEC3C reveals a binding pocket modulating ribonucleic acid interaction required for encapsidation. Proc. Natl. Acad. Sci. USA 2009. 106:12079–12084

14. Muckenfuss, H.; Hamdorf, M.; Held, U.; Perkovic, M.; Löwer, J.; Cichutek, K.; Flory, E.; Schumann, G.G.; Münk, C. APOBEC3 Proteins Inhibit Human LINE-1 Retrotransposition. J. Biol. Chem. 2006. 281:22161–22172.

15. Marino, D.; Perkovic, M.; Hain, A.; Vasudevan, A.A.J.; Hofmann, H.; Hanschmann, K.-M.; Mühlebach, M.D.; Schumann, G.G.; König, R.; Cichutek, K.; et al. APOBEC4 Enhances the Replication of HIV-1. PLoS ONE 2016, 11, e0155422.

16. Schüle S, Kloke B-P, Kaiser JK, Heidmeier S, Panitz S, Wolfrum N, Cichutek K, Schweizer M. 2009. Restriction of HIV-1 replication in monocytes is abolished by Vpx of SIVsmmPBj. PLoS One 4:e7098. https://doi.org/10.1371/journal.pone.0007098

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