Gary B Ruvkun contribution to E. coli Plasmid and understanding of Endotoxin Lipid A supertoxicity

2024 Nobel Prize sharer in Physiology or Medicine helped make all of the Jab mass production and GMO technology possible through his manipulation of Bacteria

Please see related article on Victor Ambros.¹

Gary B Ruvkun was a pioneer in use of E. coli to produce mutations by inserting transposons into circular DNA known as Plasmid.²

Interesting that his 1981 paper in Nature is still behind a paywall.

He used Sinorhizobium meliloti or Rhizobium meliloti Gram-negative bacteria that have had a name change to Ensifer meliloti.³ These bacteria have a Symbiotic relationship with many Legumes and are important in Nitrogen Fixation.

In 2003, German researchers took a close look at the structure of Sinorhizobium meliloti Lipid A and found the C28 fatty acid moiety of lipid A is not crucial, but it is beneficial for establishing an effective symbiosis with host plants..⁴

Readers will be aware that the US Military spend a large amount of effort in manipulating Endotoxin Lipid A.⁵ ⁶

TGA could but chooses not to measure Lipid A.⁷⁸

Novavax is inspired.⁹ GSK implements modified Lipid A.¹⁰ Nikolai Petrovsky is keen on Endotoxin adjuvants.¹¹ Moderna Lipid A is different to Pfizer’s.¹²

Ruvkun’s Nobel Prize award is not about his GMO manipulation of living things and their toxins, but rather his collaborative work on the Epigenetic effects of microRNAs with Ambros.

The Nobel Prize citation¹³ states:

The published results were initially met with almost deafening silence from the scientific community. Although the results were interesting, the unusual mechanism of gene regulation was considered a peculiarity of C. elegans, likely irrelevant to humans and other more complex animals.

Such is the lack of imagination of people who regulate GMOs!

1.

2. Gary B. Ruvkun and Frederick M. Ausubel. 1981. A general method for site-directed mutagenesis in prokaryotes. https://www.nature.com/articles/289085a0

3. https://en.wikipedia.org/wiki/Ensifer_meliloti

4. Larissa A. Sharypova, Karsten Niehaus, Heiko Scheidle, Otto Holst, Anke Becker. 2003. Sinorhizobium meliloti acpXL Mutant Lacks the C28 Hydroxylated Fatty Acid Moiety of Lipid A and Does Not Express a Slow Migrating Form of Lipopolysaccharide. https://www.sciencedirect.com/science/article/pii/S0021925819646673

5. Peter Hotez promotes Endotoxin and Neurotoxic Aluminium in Jabs

6. Devilish Details of Endotoxin in LNPs

7. Lipid A in Jabs, the Supertoxin they won't report

8. Meningitis caused by Endotoxin Lipid A in Covid19 Jabs

9. NovaVax preferred Adjuvants include Endotoxin Lipid A

10. GSK proprietary AS01 Endotoxin Liposomes are more toxic due to DOPC

11. SpikoGen formerly Covax-19 update

12. Moderna DH5α Escherichia coli Bacteria Endotoxin and Lipid A - what do we know?

13. https://www.nobelprize.org/prizes/medicine/2024/press-release/