In November 2023 I told my Substack readers that Robert Malone is an expert in Endotoxin.1
Photo source is his X account where he has 1.3 Million followers.2
So this is just a brief reminder pointing to how useful his knowledge is in relation to Sudden Death Anaphylaxis and other Jab Harms caused by the inevitable or deliberately added supertoxin.
Here is a snip from his October 2023 article3 that I cited.
The paper he wrote4 with Phillip Montbriand in 1995 on an improved method of Endotoxin (LPS) removal from DNA using Polymyxin B resin was of great interest to researchers wanting to investigate the confounding effects of residual contaminant.
It is still behind a paywall, cited in 28 other papers.
Here are a few quotes:
subnanogram quantities of LPS can alter the phenotype of various cell types (Gould, 1984). Affected cell types include mononuclear, endothelial, and smooth muscle cells as well as polymorphonuclear granulocytes and thrombocytes. Macrophages and monocyctes are known to be particularly responsive to LPS (Galanos et al., 1992), and responds to LPS stimulation by producing bioactive lipids, reactive oxygen species, and various peptide inflammatory mediators (Rietschel et al., 1994).
In vivo administration of LPS during the course of acute inflammation is also known to result in elevated systemic levels of TNF-α, MIP-2, IL-lβ, and IL-6 (Xing et al., 1994).
Therefore, treatment of animals or cultured cells with plasmid DNA which is contaminated with LPS may introduce artifacts which are a consequence of unintentional LPS treatment.
Robert Malone annd his colleague encountered problems using Polymixin, as shown in their Table 2.
Further attempts to remove LPS from solutions containing DNA after the initial purification were unsuccessful. A baseline reactivity of the LAL assay remained after four passages.
We hypothesize that the baseline LAL reactivity whch was observed represents the cross-reactivity of DNA in the LAL test.
A 2024 paper5 citing Montbriand and Malone shows that Endotoxin is still a confounder in production of long single-stranded DNA. As the authors say:
Although we purify the ssDNA from the phage proteins using ethanol purification, endotoxins can be retained with DNA in alcohol-based DNA precipitation methods (Montbriand and Malone 1996).
We suspect that additional residual endotoxins from bacterial production of the templates may cause cell death, contributing to the decreased knock-in efficiency.
Polymixin becomes a contaminant in Jabs when it is used, as I pointed out for Wyeth Dryvax Smallpox vaccine6 which caused huge numbers of Adverse Reactions in both Military and Civilian recipients, Monkey Pox7 and high lethality childhood Jabs Infanrix8 and Vaxelis.9
It can also be involved in Kidney Dialysis and attempts to rescue people suffering Endotoxin Sepsis.1011
Profits are severely reduced by protein losses during passage through polymyxin B columns, so you will find few manufacturers using the Montbriand and Malone method. In addition the BioNTech patent made it absolutely clear that Endotoxin and its Lipid A are required in all Pfizer Jabs as “adjuvant”.
Late Migrating Species in Pfizer Jabs caused by Endotoxin
Recently Dr Robert W Malone reminded his readers that in 1995 he and colleague Phillip M Montbriand developed a simple technique to remove most of the Endotoxin contamination from the DNA with which they were experimenting because it would ruin the chance of proper analysis of transfection and toxicology if left in.
https://x.com/RWMaloneMD
Phillip M. Montbriand and Robert W. Malone. 1996. Journal of Biotechnology 44:43-46. https://www.sciencedirect.com/science/article/abs/pii/0168165695000917
Konlin Shen, Jake J. Flood, Zhihuizi Zhang, Alvin Ha, Brian R. Shy, John E. Dueber and Shawn M. Douglas. 2024. Engineering an Escherichia coli strain for production of long single-stranded DNA. Nucleic Acids Research , 2024:1–10 https://doi.org/10.1093/nar/gkae189
Smallpox vaccine Dryvax contained Live Bacteria as well as Endotoxin
Previously I wrote about Pfizer purchasing Wyeth in its decades long plan to become the dominant player in Jabbing the World.
Monkeypox Jab ACAM2000 contains Endotoxin scavenger Polymyxin B
Why would manufacturers add “trace amounts” of Neomycin and Polymyxin B to ACAM2000 ?
Vaxelis Endotoxin content a Dark TGA Secret
Looking for any information at all on the Endotoxin of Vaxelis jabs approved by the TGA in March 2022. I know it is in there.
Shedding Light on your Blood Endotoxin Level
My Chemistry students favourite laboratory practical was the joy of making Luminol Chemiluminescence.
Saturday Endotoxin weekly Roundup VIII
In a 2002 paper by Air War Colonel Michael Ainscough of the USAF called "New Generation Bioweapons - The Technology of Genetic Engineering Applied to Biowarfare and Bioterrorism" he cited Varicella Virus which reactivates as Herpes Zoster (Shingles) as an example of a Stealth Weapon.
Quite a few people still mistrust Dr. Malone, but I think because he spoke out against his own invention, was removed from his own wikipage, and then blamed for each and every word he published, he has to be trustworthy. I also enjoy his farming pages immensely.