SV40 sequence in Novavax ? - Who is measuring it in vile vials?

Please help me find measurements of SV40 contamination in Novavax vials

I was just looking at a few documents I have on the Novavax Covid19 Jab and wondered why there seems to be so little discussion of the GMO Plasmid structure where Simian Virus SV40 leaps out at us.

The caption to the figure was fuzzy, but here it is:

The description reads:

The pBAC transfer plasmid pNVAX5032 (typo by the vendor, should be PNVAX4032) containing the SARS-CoV-2 rS gene and flanking sequences is shown in Figure 3.2.S.2.3.4.

Beginning at 12 o'clock position on the plasmid and proceeding clockwise are the following structural elements: E.coli plasmid FI and Lef2, the baculovirus ORF603 gene required to rescue the flaskBacGOLD baculovirus DNA in transfected Sf9 cells as described in Section 3.2.S.2.3.3.3 Transfection of Recombinant Baculovirus Generation (PO), the AcMNPV baculovirus polyhedron promoter (polH) that controls the transcription of the rS gene (nCov-2019/QQAQ/K986P/V987P) in Sf9 cells, followed by an mRNA polyadenylation signal (SV40polyA), homologous AcMNPV gene sequence, and flanking the rS gene Bglll/BamHI and Hindlll restriction enzyme sites to assist in cloning the gene into the pNvax4032 E.coli transfer plasmid.

Inserted in the resulting recombinant AcMNPV baculovirus BV2373 is the rS gene under transcriptional control of the polyhedron promoter and repaired adjacent ORF603 gene required to rescue the FlashBacGOLD baculovirus DNA.

I searched X and found only a few people asking about this sequence and now the search is on for the full 9,308 base pairs.

Here is more information from the manufacturer.¹²

Please send me links to any data you find.

My earlier posts on the Novavax Jab.³⁴⁵⁶

Update

Since requesting information, Kevin McKernan communicated via our email discussion group, where he said:

I don’t see any SV40 promoters/Enhancers or Origins of replication in their plasmid.

Only an SV40 polyA signal which is a transcription termination signal.

I have subsequently learned that the displayed diagram submitted for authorization shows polH which stands for the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) polyhedron promoter, specifically the p10 promoter, which is described as a crucial element for late gene expression and is used for driving the expression of foreign genes in baculovirus systems.

Learning as I go, found a number of patents⁷ where Novavax includes the use of early and late SV40 promoters, but it appears they are not used in their Covid19 Jab.

SV40 polyA tail can’t be described as “safe”

The effects of the SV40 polyA sequence are of interest, sometimes producing higher-molecular-mass gene fusion RNA.⁸

A quick search of PubMed for “SV40 polyA” yields 137 papers.⁹

Skimming through the titles and abstracts reveals discovery of unintended consequences. Terms used include interruption of gene expression, overexpression, nonsense, antisense, premature truncation, wraparound transcription.

Time for more input from genomics experts on dangers of this GMO Jab.

1. https://x.com/AI2BO4/status/1713644203685658864/photo/1

2. https://x.com/vegananne/status/1883554431901569251

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7. Gale Smith, Rick Bright, Peter M. Pushko, Jinyou Zhang, Kutub Mahmood. Functional influenza virus-like particles (vlps). Expired patent. US20190038738A1. https://patents.google.com/patent/US20190038738A1/en

8. https://pubmed.ncbi.nlm.nih.gov/22306880/

9. https://pubmed.ncbi.nlm.nih.gov/?term=SV40+polyA