Pfizer knew how to remove Double Stranded RNA Contaminants in 2011 but didn't do it for Covid19

Kevin McKernan, Phillip Buckhaults and Prof. Dr. Brigitte König MMD labs independently analyzed Pfizer jabs and proved contamination above arbitrary "limits". Pfizer saved money by bottling toxic soup

Just a quick post today on how Pfizer knew how to purify its modified mRNA in 2011 and boasted about it. But when it came to jabbing billions of doses into the unsuspecting global population, they failed to use their own proven technology of High Performance Liquid Chromatography (HPLC) and instead used crude filtration.

Here is a figure from 2011 that shows the dramatic reduction in Inflammatory Cytokines achieved with HPLC.¹

They used Triphosphate-derivatives of pseudouridine (Ψ) and 5-methylcytidine (m5C) (TriLink) to generate modified nucleoside containing RNA.

They studied the inflammatory generation of IFN-α, IFN-β, or TNF-α.

Independent contamination proof

Kevin McKernan, Phillip Buckhaults and Prof. Dr. Brigitte König MMD labs independently analyzed Pfizer jabs and proved contamination above arbitrary "limits".

Industrial Scale HPLC is available but expensive

As I reported in an earlier post², large scale HPLC is available for Endotoxin removal and cleaning up materials grown in Bacteria to reduce Cytokine Storm³, but of course that would have cost Pfizer BioNTech a lot of money.⁴

Pfizer chose not to use a Cheap Purification

In April 2019, BioNTech published a very Cheap method of removing dsRNA contaminant from their mRNA jabs.⁵ Of course they did not use that in manufacture of their Process 2 jabs because it would have reduced yield and therefore Profit.

I wonder what you think about that?

Will add more later.⁶

January Update

Thanks to my friend Kevin McKernan who discovered a recently published US Patent Application by BioNTech further emphasizing the filthy E. coli used in jab production leaves Endotoxin and plasmid DNA.

A quote from the text, which is freely⁷ available:

In some embodiments, a plasmid DNA may be obtained, e.g., from bacterial cells (e.g., Escherichia coli (E. coli)) followed by an Endotoxin and animal product-free plasmid isolation procedure.

Download the BioNTech Pending Patent Application as a pdf.⁸

1. Katalin Kariko, Hiromi Muramatsu, Janos Ludwig and Drew Weissman. 2011. Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein-encoding mRNA. Nucleic Acids Research, 2011, Vol. 39, No. 21 e142

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5. Markus Baiersdörfer, Gábor Boros, Hiromi Muramatsu, Azita Mahiny, Irena Vlatkovic, Ugur Sahin and Katalin Karikó. 2019. A Facile Method for the Removal of dsRNA Contaminant from In Vitro-Transcribed mRNA. https://www.cell.com/molecular-therapy-family/nucleic-acids/fulltext/S2162-2531(19)30049-6

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7. Irena Rabe, Maximilian Buff, Thomas Ziegenhals, Johanna Drögemüller, Andreas Kuhn, Stephanie Fesser, Rodney Gene Combs, Nicole Eschmann; Jennifer Ann Schoborg Romine, Jenna Kathryn Williamson. 2022. US Patent US20230183769A1 Pending. In vitro transcription technologies. https://patents.justia.com/patent/20230183769

8. Irena Rabe, Maximilian Buff, Thomas Ziegenhals, Johanna Drögemüller, Andreas Kuhn, Stephanie Fesser, Rodney Gene Combs, Nicole Eschmann; Jennifer Ann Schoborg Romine, Jenna Kathryn Williamson. 2022. US Patent US20230183769A1 Pending. In vitro transcription technologies. https://patents.google.com/patent/US20230183769A1/en?oq=US20230183769A1