Endotoxin Jabbing of Wild Finches in Estonia relates to Lung Cancer from Smoking
25 Million years Evolutionary divergence prompted Estonian researchers to capture Wild Green Finches, jab them with Endotoxin, draw Whole Blood, and use Genomic sequences identifying Genes affected.
I am currently working on a document to be submitted to a number of goverments, including Estonia, on the Adverse Reactions of mRNA Jabs.
I saw a Substack article1 by my friend, Endotoxin measurer2 and Genomic Sequencing dynamo Kevin McKernan, where he mentioned one of his papers looking at Somatic Mutations induced in Human lungs by Tobacco smoke where the CHD7 Gene turned out to be a useful marker.3
That reminded me of a later paper by Richard Meitern and coworkers in Estonia who found that CHD7 Gene was one of a very large number of genes differentially expressed in captured wild Female Green Finch (Carduelis chloris) Blood, twelve hours after Endotoxin (LPS) jab.4 Note the Genomicist jargon in the figure caption.
They were fed up with published studies using inbred Mice, or even Humanized Mice for Endotoxin jabbing experments.
Four Finches received an injection of 0.1 mg E. coli Endotoxin (LPS strain 055:B5, Sigma L2880) in 40 μL sterile isotonic saline into the pectoralis muscle. The remaining four Finches received 40 μL isotonic saline injections.
They found:
LPS-injected birds lost significantly more body weight (change in body mass −0.6 ± 0.3 g in LPS injected birds vs 0.4 ± 0.4 g in saline injected birds, t = 3.95, df = 6, p = 0.008), and the experimental procedure significantly affected 1,911 transcripts (absolute fold change >2, Baggerley's test P-value <0.01), of which only 466 (420 unique genes) had been successfully annotated using Uniprot-SwissProt.
Here is their pie chart admitting they are clueless about the effects that many of the altered expressions would have on the Finches or their offsprng when they were subsequently released to the wild.
They do state that Plumage coloration of greenfinches is sensitive to infections.
You have to download the supplementary spreadsheet file to fully appreciate the impact of injected Endotoxin on the Finches.
CHD7 is known by many names, making literature search tedious, and has its expression changed by toxins.5
Should Endotoxin Jabbing be done on Wildlife
Do you think the Ethics approval of the Committee of Animal Experiments at the Estonian Ministry of Agriculture was justified?
Erin D. Pleasance, Philip J. Stephens, Sarah O’Meara, David J. McBride, Alison Meynert, David Jones, Meng-Lay Lin, David Beare, King Wai Lau, Chris Greenman, Ignacio Varela, Serena Nik-Zainal, Helen R. Davies, Gonzalo R. Ordoñez, Laura J. Mudie, Calli Latimer, Sarah Edkins, Lucy Stebbings, Lina Chen, Mingming Jia, Catherine Leroy, John Marshall, Andrew Menzies, Adam Butler, Jon W. Teague, Jonathon Mangion, Yongming A. Sun, Stephen F. McLaughlin, Heather E. Peckham, Eric F. Tsung, Gina L. Costa, Clarence C. Lee, John D. Minna, Adi Gazdar, Ewan Birney, Michael D. Rhodes, Kevin J. McKernan, Michael R. Stratton, P. Andrew Futreal Peter J Campbell. 2009. A small-cell lung cancer genome with complex signatures of tobacco exposure. https://www.nature.com/articles/nature08629
Richard Meitern1, Reidar Andreson and Peeter Hõrak. 2014. Profile of whole blood gene expression following immune stimulation in a wild passerine. BMC Genomics 15:533. http://www.biomedcentral.com/1471-2164/15/533
https://ctdbase.org/detail.go?type=gene&acc=55636
This is outrageous. All these labs that experiment on animals should be closed for good. Animals are not to suffer for the pleasure or the money making of humans. If they want to experiment, do it as in olden times. Inject themselves. Leave the wild birds and animals (and all the other animals) alone. And the unwilling humans as well!
It's sad what otherwise compassionate people do to animals under the guise of greater good. The factory farms have and deserve the appalling name they had but the laboratories appear to be just as bad.