David Speicher collaboration with Wuhan Institute of Virology on sequencing Covid19

David did much of his research at Griffith University where he obtained his Masters and PhD degrees looking at Human Coronaviruses. Just discovered he is an Endotoxin expert as well.

Update

This article has been corrected to remove my erroneous inclusion of work by another researcher named David W Speicher.

Apologies to David J Speicher who communicates directly with me via X and pointed out my error while I was sleeping.

Wuhan paper

The paper I dug out was published in August 2021 by David J Speicher and gave a detailed procedure¹ for use of magnetic beads to capture “Bait”.

His collaborators were:

• Jalees A Nasir from McMaster University

• Peng Zhou from CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China, and

• Danielle E Anderson from Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore.

Here is their Figure outlining the steps.

Fig. 1 The pre-sequencing target-enrichment workflow including blocking, hybridization, and isolation of viral sequences

David published a paper² on Human Coronavirus in 2006 when he was working at Queensland Paediatric Infectious Diseases Laboratory, CVRU, Sir Albert Sakzewski Virus Research Centre, Royal Children’s Hospital, Herston.

His co-authors were Theo P Sloots, Peter McErlean, Katherine E Arden, Michael D Nissen and Ian M Mackay.

Abstract

Undiagnosed cases of respiratory tract disease suspected of an infectious aetiology peak during the winter months. Since studies applying molecular diagnostic assays usually report reductions in the number of undiagnosed cases of infectious disease compared to traditional techniques, we applied PCR assays to investigate the role of two recently described viruses, namely Human Coronavirus (HCoV) HKU1 and Human Bocavirus (HBoV), in a hospital-based paediatric population. Both viruses were found among Australia children with upper or lower respiratory tract disease during the autumn and winter of 2004, contributing to 21.1% of all microbial diagnoses, with individual incidences of 3.1% (HCoV-HKU1) and 5.6% (HBoV) among 324 specimens. HBoV was found to coincide with another virus in more than half of all instances and displayed a single genetic lineage, whilst HCoV-HKU1 was more likely to occur in the absence of another microbe and strains could be divided into two genetic lineages which we propose be termed HCoV-HKU1 type A and type B. Children under the age of 2 years were most at risk of infection by these viruses which contribute significantly to the microbial burden among patients with respiratory tract disease during the colder months.

In 2012 he published more details on Griffith University research looking at Coronaviruses circulating in Queensland children in 2004.³

Abstract

Acute respiratory illnesses (ARIs) with unconfirmed infectious aetiologies peak at different times of the year. Molecular diagnostic assays reduce the number of unconfirmed ARIs compared to serology- or culture-based techniques.

Screening of 888 inpatient and outpatient respiratory specimens spanning late autumn through to early spring, 2004, identified the presence of a Human Coronavirus (HCoV) on 74 occasions (8.3% of all specimens and 26.3% of all respiratory virus detections).

Prevalence peaked in August (late winter in the southern hemisphere) when they were detected in 21.9% of specimens tested.

HCoV-HKU1 and HCoV-OC43 comprised 82.4% of all HCoVs detected.

Positive specimens were used to develop novel reverse transcriptase real-time PCRs (RT-rtPCRs) for HCoV detection.

An objective clinical severity score was assigned to each positive HCoV patient. Severity scores were similar to those from a random selection of young children who were positive for respiratory syncytial virus at a different time but from the same specimen population.

During the cooler months of 2004, sensitive and specific RT-rtPCRs identified the concurrent circulation of all four HCoVs, a quarter of which co-occurred with another virus and most of which were from children under the age of two years.

David Speicher Endotoxin research

I knew that David has a keen interest in Bacteria and the Endotoxin they shed when he responded to Maria Gutschi suggesting she check out the excellent video made by my friend Gaz in South Australia.⁴

David published a paper on Oral Cancer related to Endotoxin in 2018 when he had joint appointments at Menzies Health Institute Queensland, Griffith University, Queensland and McMaster University, Ontario, Canada.⁵

Abstract

Results from microbiome studies on oral cancer have been inconsistent, probably because they focused on compositional analysis, which does not account for functional redundancy among oral bacteria.

Based on functional prediction, a recent study revealed enrichment of inflammatory bacterial attributes in Oral Squamous Cell Carcinoma (OSCC).

Given the high relevance of this finding to carcinogenesis, we aimed here to corroborate them in a case-control study involving 25 OSCC cases and 27 fibroepithelial polyp (FEP) controls from Sri Lanka.

DNA extracted from fresh biopsies was sequenced for the V1 to V3 region with Illumina’s 2 × 300-bp chemistry. High-quality nonchimeric merged reads were classified to the species level with a prioritized BLASTN-based algorithm. Downstream compositional analysis was performed with QIIME (Quantitative Insights into Microbial Ecology) and linear discriminant analysis effect size, while PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) was utilized for bacteriome functional prediction.

The OSCC tissues tended to have lower species richness and diversity.

Genera Capnocytophaga, Pseudomonas, and Atopobium were overrepresented in OSCC, while Lautropia, Staphylococcus, and Propionibacterium were the most abundant in FEP.

At the species level, Campylobacter concisus, Prevotella salivae, Prevotella loeschii, and Fusobacterium oral taxon 204 were enriched in OSCC, while Streptococcus mitis, Streptococcus oral taxon 070, Lautropia mirabilis, and Rothia dentocariosa among others were more abundant in FEP.

Functionally, proinflammatory bacterial attributes, including Lipopolysaccharide biosynthesis and peptidases, were enriched in the OSCC tissues.

Thus, while the results in terms of species composition significantly differed from the original study, they were consistent at the functional level, substantiating evidence for the inflammatory nature of the bacteriome associated with OSCC.

In the discussion you can find how important the authors considered their new discoveries where captalize and use bold for emphasis:

More important, the study performed functional prediction analysis, which revealed enrichment of proinflammatory bacterial attributes, such as Lipopolysaccharide (LPS, Endotoxin) synthesis, Flagellar assembly, and Bacterial Chemotaxis in the tumor tissue.

This is a very important finding given the well-established role of inflammation in Cancer, but it has not been confirmed by another study.

In public discussion ongoing via X, I pointed out to David 270 papers⁶ found on PubMed when searching “Oral Cancer Endotoxin” and I suggested that Endotoxin experts at University of Guelph⁷ might be able to help arrange Endotoxin measurements of different Lots of Covid19 Jab.⁸

“Oral Cancer LPS” returns 362 papers.⁹

PubMed when searching “Oral Cancer Lipopolysaccharide” finds 429 papers.¹⁰

David’s co-authors and Designer Covid19

Recently I was introduced to the outstanding work of Jim Haslam exposing the US Bioweapon work funded by Fauci through DARPA grants.¹¹

I was searching for Peng Zhou and Danielle Anderson (known as Dani) on X because I had seen their names mentioned frequently, and now it starts to make more sense.

I strongly recommend a massive thread on X posted by Jim Haslam on 25 October 2025 that appears to have a small number of reads despite beng shared 144 times.¹²

Here I have borrowed some of his slides relevant to David Speicher’s co-authors.

In 2016 Peng Zhou of Wuhan was at a strategy meeting with his boss frequently known as “Bat Lady” Shi Zhengli.¹³

Bat Lady and Peng Zhou published the full genomic sequence in 2006 in collaboration with Meng Yu of Australia’s CSIRO Livestock Industries, Australian Animal Health Laboratory at Geelong in the state of Victoria.¹⁴

Peng Zhou trained in Australia

According to an article¹⁵ published on 9 February 2020

Dr Shi’s protégé, Peng Zhou — now the head of the Bat Virus Infection and Immunity Project at the Wuhan Institute of Virology — spent three years at the bio-containment facility Australian Animal Health Laboratory between 2011 and 2014.

He was sent by China to complete his doctorate at the CSIRO from 2009-2010.

During this time, Dr Zhou arranged for wild-caught bats to be transported alive by air from Queensland to the lab in Victoria where they were euthanised for dissection and studied for deadly viruses.

Dr Linfa Wang, while an Honorary Professor of the Wuhan Institute of Virology between 2005 and 2011, also worked in the CSIRO Office of the Chief Executive Science Leader in Virology between 2008 and 2011.

DARPA Defuse project

The Coronavirus project DEFUSE proposal requested US$14,209,245 over 3 and a half years.

In March 2018 we see some names of those involved in emails from Hongying L to Peng Zhou and Bat Lady re their revamped PREEMPT grant application.

Ben Hu, Noam Ross, Luke Hamel, Alice Latinne

and

In June 2018 Danielle Anderson expressed her disappointment that their Coronavirus project PREEMPT proposal had not received funding.

Note Ralph Baric, Amy Sims, Timothy Sheahan, Shi Zhengli, Peng Zhou, Lin-Fa Wang, Aaron Trent Irving, Rocke, R Abbott, Peter Daszak, Alexsei Chmura, Kevin Olival, Evelyn Luciano, Hongying Li, Alison Andre among the recipients.

As Jim Haslam points out, they produced a new proposal and 2 grants to divide the work, both awarded by Fauci.

Cell phone tracking has been used to see where individuals travelled before Wuhan.

Peng Zhou wrote to Peter Daszak on 14 November 2020 regarding DARPA project DEFUSE.

Jim Haslam tells us cell phone tracking has been used to see where individuals travelled before Wuhan.

Please share if you find this history interesting and let me know if some of the names ring a bell.

1. David J Speicher, Jalees A Nasir, Peng Zhou, Danielle E Anderson. 2021. Whole-Genome Sequencing of Pathogens in Saliva : A Target-Enrichment Approach for SARS-CoV-2. Methods Mol Biol. 2327:119-137. doi: 10.1007/978-1-0716-1518-8_8. https://link.springer.com/protocol/10.1007/978-1-0716-1518-8_8

2. Theo P Sloots, Peter McErlean, David J Speicher, Katherine E Arden, Michael D Nissen, Ian M Mackay. Evidence of human coronavirus HKU1 and human bocavirus in Australian children. J Clin Virol. 35(1):99-102. doi: 10.1016/j.jcv.2005.09.008.

3. Ian M Mackay, Katherine E Arden, David J Speicher, Nicholas T O’Neil, Peter K McErlean, Ristan M Greer, Michael D Nissen, Theo P Sloots. 2012. Co-circulation of four human coronaviruses (HCoVs) in Queensland children with acute respiratory tract illnesses in 2004. Viruses. 4(4):637-53. doi: 10.3390/v4040637.

4.

5. Manosha Perera, Nezar Noor Al-Hebshi, Irosha Perera, Deepak Ipe, Glen C Ulett, David J Speicher, Tsute Chen, Newell W Johnson. 2018. Inflammatory Bacteriome and Oral Squamous Cell Carcinoma. J Dent Res. 97(6):725-732. doi: 10.1177/0022034518767118.

6. https://pubmed.ncbi.nlm.nih.gov/?term=oral+cancer+endotoxin

7.

8. https://x.com/FluoridePoison/status/1994209032899264716

9. https://pubmed.ncbi.nlm.nih.gov/?term=oral+cancer+LPS

10. https://pubmed.ncbi.nlm.nih.gov/?term=oral+cancer+lipopolysaccharide

11. 293 Questions for Ralph Baric

12. https://x.com/jhas5/status/1982104267814252596

13. https://en.wikipedia.org/wiki/Shi_Zhengli

14. Ren, Wuze; Li, Wendong; Yu, Meng; Hao, Pei; Zhang, Yuan; Zhou, Peng; Zhang, Shuyi; Zhao, Guoping; Zhong, Yang; Wang, Shengyue; Wang, Lin-Fa; Shi, Zhengli. 2006. Full-length genome sequences of two SARS-like coronaviruses in horseshoe bats and genetic variation analysis J Gen Virol. 87 (11): 3355–3359.

15. Narendar Singh. 9 February 2020. Coronavirus NSW: Dossier lays out case against China bat virus program. https://www.strategicfront.org/forums/threads/chinese-wuhan-virus-thread.3819/page-126